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The various subtypes of avian influenza virus like H5, H6 and H9 are increasing in many areas of the world. In various parts of the world, birds are vaccinated against AIV, using inactivated vaccines, subunit vaccines or recombinant vector vaccines. The diagnostic approach has to be adapted to the local situation, preferably using the Differentiating Infected from Vaccinated Animals (DIVA) strategy. This presentation will give an update on the aspects and developments in the detection of AI infections.